By Jose M Martinez-Zapater, Julio Salinas

This accomplished choice of present and crucial protocols includes many simply reproducible tools constructed to be used with Arabidopsis - a approach for impending basic questions in plant biology. The equipment diversity from the fundamentals of growing to be those vegetation to stylish gene cloning techniques and will, in lots of circumstances, even be utilized to different plant species with minor changes. Sections on genetics, transformation and gene expression research which are in particular necessary to scientists all for mutant research or generating and reading transgenic crops.

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Extra info for Arabidopsis Protocols (Methods in Molecular Biology (Cloth))

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Plant Physzol. 106, 159-164 9 Preparation of DNA from Arabidopsis Jianming Li and Joanne Chory 1. Introduction Successful extraction of DNA is an essential and time consuming step in many plant molecular biology procedures. The classical approaches of plant DNA isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols are aimed at rapid extraction of a minute quantity of plant DNA that 1ssultable for PCR.

8 Murashige, T. and Skoog, F (1962) A revised medmm for rapid growth and bioassays with tobacco tissue cultures Phystol Plant 15,473+97. 9 Gamborg, 0. L , Miller, R A and OJtma, K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50, 15 l-158 5 Callus Culture and Regeneration Jaideep Mathur and Csaba Koncz 1. Introduction Regeneration of plants by micropropagation of in vitro cultures can be achieved from organ primordia existing m shoot tips and axillary bud explants.

Plant Physzol. 106, 159-164 9 Preparation of DNA from Arabidopsis Jianming Li and Joanne Chory 1. Introduction Successful extraction of DNA is an essential and time consuming step in many plant molecular biology procedures. The classical approaches of plant DNA isolation are often designed to produce large amounts of DNA of high molecular weight with sufficient purity. Since the polymerase chain reaction (PCR) 1s the method of choice in modern plant genetic analyses such as map-based breeding and positional gene clonmg, many recently developed protocols are aimed at rapid extraction of a minute quantity of plant DNA that 1ssultable for PCR.

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