By Jesse P. Greenstein (Auth.)

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Both groups of investigators used mice as test objects and in the discussion of the carcinogenic activity of the polycyclic hydrocarbons in this connection it is understood that it is the susceptibility of mice toward these agents which is under consideration. The London group was faced with the problem of examining the carcinogenicity of literally hundreds of synthetic polycyclic hydrocarbons and their derivatives. 3% solution of the test compound in benzene solution. When tumors resulted from this painting, they were examined histologically and ordinarily found to be epitheliomas (skin tumors).

16; 19), and the possibility that sesame oil might be carcinogenic too was pointed out by Gardner (20). Even if these vehicles were not carcinogenic, such complex fats as lard and sesame oil are rarely reproducible in chemical constitution and in properties from one preparation to another. The question of whether the vehicle, though not carcinogenic itself, yet may affect the carcinogenic potency of a given hydrocarbon, was considered by many investigators (21; 22). This problem has wide ramifications and is discussed below in a later section (p.

That lard itself might have carcinogenic properties was recognized by Burrows et al. (16; 19), and the possibility that sesame oil might be carcinogenic too was pointed out by Gardner (20). Even if these vehicles were not carcinogenic, such complex fats as lard and sesame oil are rarely reproducible in chemical constitution and in properties from one preparation to another. The question of whether the vehicle, though not carcinogenic itself, yet may affect the carcinogenic potency of a given hydrocarbon, was considered by many investigators (21; 22).

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