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Huzyk and D. J. Clark, J. Bacteriol. 108, 74 (1971). 31 I. Vlodavsky, M. Inbar, and L. Sachs, Proc. Natl. Acad. Sci. A. 70, 1780 (1973). 31 R. Wahl and L. M. Kozloff, J. Biol. Chem. 237, 1953 (1962). 33 E. Egberts, P. B. Hackett, and P. Traub, J. Virol. 22, 591 (1977). 34 y . Tsuchiya and H. Sugai, Biochem. Med. 28, 256 (1982). 35 p. McWalter, J. Appl. Bacteriol. 56, 145 (1984). 36 R. Guerrero, M. Llagostera, A. Villaverde, and J. Barb6, J. Gen. Microbiol. 130, 2247 (1984). 37 G. E. Thomas, S. Levitskyy, and H.

Since formation of ATP during extraction is unlikely, the high yield with TCA strongly indicates that this is the most reliable extractant. A high yield of ADP and AMP or even the sum of all three adenine nucleotides may be obtained with poor extractants, since ADP and AMP may be formed by degradation of other cell constituents. Figure 1 shows results from an experiment of the type described above performed with LNCaP-r cells dispersed with Na-citrate and suspended in fresh medium. In the experiment (performed at room temperature) a 100-/zl cell suspension was added to 900/zl of extractant.

Lewenstein and R. Bachofen, Arch. Microbiol. 116, 169 (1978). 18 C. M. M. Franco, J. E. Smith, and D. R. Berry, J. Gen. Microbiol. 130, 2465 (1984). 19 M. B. Nair and R. R. Eady, J. Gen. Microbiol. 130, 3063 (1984), 2o R. E. Strange, H. E. Wade, and F. A. Dark, Nature (London) 199, 55 (1963). 21 R. R. Mathis and O. R. Brown, Biochem. Biophys. Acta 440, 723 (1976). 22 y. N. Lee and M. J. Colston, J. Gen. Microbiol. 131, 3331 (1985). 23 K. D. Beaman and J. D. Pollack, J. Gen. Microbiol. 129, 3103 (1983).

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