By Zhenjiang Li, Xiaojun Ji, Suli Kan, Hongqun Qiao, Min Jiang (auth.), G. T. Tsao, Pingkai Ouyang, Jian Chen (eds.)
Past, current, and destiny commercial Biotechnology in China, via Zhenjiang Li, Xiaojun Ji, Suli Kan, Hongqun Qiao, Min Jiang, Dingqiang Lu, Jun Wang, He Huang, Honghua Jia, Pingkai Ouyuang, and Hanjie Ying; natural chemical substances from Bioprocesses in China, by way of Jin Huang, Lei Huang, Jianping Lin, Zhinan Xu, and Peilin Cen; Biofuels in China, via Tianwei Tan, Jianliang Yu, Jike Lu, and Tao Zhang; Bioreactors and Bioseparation, through Siliang Zhang, Xuejun Cao, Ju Chu, Jiangchao Qian, and Yingping Zhuang; Environmental Biotechnology in China, by means of Shuang Jiang Liu , Lei Liu , Muhammad Tausif Chaudhry , Lei Wang , Ying Guang Chen , Qi Zhou , He Liu , and Jian Chen; conventional chinese language Biotechnology, via Yan Xu , Dong Wang , Wen Lai Fan , Xiao Qing Mu, and Jian Chen; sleek Biotechnology in China, through Qing-Zhao Wang and Xue-Ming Zhao
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Substantial time and effort is allotted to introducing cellphone tradition and fermentation expertise to undergraduate scholars in academia, usually via quite a number classes in business biotechnology and comparable disciplines. equally, quite a few textbooks can be found to explain the appli- tions of those applied sciences in undefined.
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Additional info for Biotechnology in China II: Chemicals, Energy and Environment
Since cellulase was found about 100 years ago, researchers have constantly screened microorganisms degrading cellulose from soils, hot springs, oil wells, compost, and deadwood. Generally, the cellulase activity of microorganisms screened from nature is low and not suitable for industrial production. Improvement of cellulase-producing strains has received a great deal of attention from Chinese scientists. For example, an anticatabolite repression strain by mutation and capable of using 2-deoxyglucose as product inhibitor is obtained.
After screening, the recombinant P. pastoris strain Gp2025 was obtained and fermented in 25-mL methylotrophic culture medium. 00 U/mL. Even now, there are still some limitations in recombinant cellulase. Not all the genes of cellulase can be overexpressed, and recombinant cellulase lack of combined protein cannot hydrolyze the crystal cellulose. Accordingly, the topic of how to use cellulase effectively will still be a challenge in the future. 2 Industrial Cellulase Production in China Cellulase is generally produced by solid state fermentation and liquid state fermentation.
05 kDa, respectively, and it was composed of 39–40 lysine monomers. Synthesis and degradation simultaneously occurred in the production of e-PL, where the latter was caused by a e-poly-L-lysine-degrading enzyme (PLD), so studies on the properties of PLD are very important to ensure the normal fermentation and high yield of e-PL. The PLD enzyme was obtained by purification via three-step anionic ion exchange chromatography using DEAE-Sepharose, Source 15Q, and Mono Q, and was studied for its enzymological properties [48, 49].