By Victor P. Bulgakov, Yuri N. Shkryl (auth.), Pauline M Doran (eds.)
Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik, Tatiana Y. Gorpenchenko and Yuliya V. Vereshchagina: contemporary Advances within the figuring out of Agrobacterium rhizogenes-Derived Genes and Their results on tension Resistance and Plant Metabolism. Le Zhao, man W. Sander and Jacqueline V. Shanks: views of the Metabolic Engineering of Terpenoid Indole Alkaloids in Catharanthus roseus bushy Roots. Jian Wen Wang and Jian Yong Wu: potent Elicitors and strategy concepts for Enhancement of Secondary Metabolite construction in bushy Root Cultures. Amanda R. Stiles and Chun-Zhao Liu: bushy Root tradition: Bioreactor layout and approach Intensification. Marina Skarjinskaia, Karen Ruby, Adriana Araujo, Karina Taylor, Vengadesan Gopalasamy-Raju, Konstantin Musiychuk, Jessica A. Chichester, Gene A. Palmer, Patricia de l. a. Rosa, Vadim Mett, Natalia Ugulava, Stephen J. Streatfield and Vidadi Yusibov: furry Roots as a Vaccine construction and supply procedure. Zahwa Al-Shalabi and Pauline M. Doran: steel Uptake and Nanoparticle Synthesis in furry Root Cultures.
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Extra info for Biotechnology of Hairy Root Systems
MFA in one organism determines the phenotype  or cell physiology . Comparison of fluxes between phenotypes is of the utmost Table 4 MS/MS fragmentation patterns of C. roseus TIAs Alkaloid MS/MS fragmentation pattern  Precursor ion [M ? H]+ (m/z) Main fragment ion (m/z) Ajmalicine Serpentine Catharanthine 19S-Vindolinine Vindolinine Vindolidine Tabersonine Vindoline Vinblastine Vincristine 353 349 337 337 337 427 337 457 811 825 222; 317; 174; 320; 320; 409; 323; 439; 793; 807; 210; 289; 144 308 308 367; 305; 397; 751; 765; 178; 144; 117 263 158; 143 274 188 733; 680; 649; 542; 522; 355; 337 747; 723; 705; 687 46 L.
Dashed arrows indicate uncharacterized steps Perspectives of the Metabolic Engineering of Terpenoid Indole Alkaloids 37 fungal elicitation. The mRNA levels of CrMYC1 and Str genes were enhanced after induction by JA and fungal elicitation, which suggests that CrMYC1 activates the expression of the Str gene . CrMYC2 is postulated to act upstream of ORCA2 and ORCA3, activating their transcription . ORCA2, ORCA3, CrBPF-1, CrMYC1, and CrMYC2 act as transcriptional activators for some TIA biosynthetic genes as well as for several transcription repressors for TIA biosynthesis.
In the C. roseus hairy roots system, the pathway from tabersonine to vindoline is blocked. Overexpression of enzymes in the vindoline biosynthesis pathway could be a method to increase the accumulation of valuable metabolites, such as vindoline or even vincristine and vinblastine. Overexpression of tabersonine 16hydroxylase (T16H), the first enzyme in the vindoline biosynthesis pathway starting from tabersonine, in C. roseus hairy roots under the control of a glucocorticoid-inducible promoter was studied.